Identification and Detection of Citrus Psorosis Virus…

Psorosis is one of major graft-transmissible diseases of Citrus; the virus was reported to occur in different Citrus cultivated areas and constitutes a serious threat to Citrus establishment and development. This study focuses on the identification of Citrus Psorosis Virus (CPsV) by tow diagnostic methods; biological indexing in indicator seedling and serological test (DAS-ELISA). Thereby, different varieties of sweet orange (Citrus sinensis (L.) Osbeck), mandarin (Citrus × Deliciosa), lemon (Citrus × limon), Grapefruits (Citrus × Paradisi), clementine (Citrus × Clementine) and tow rootstocks sour orange (Citrus × aurantium L.) and trifoliate orange (Poncirus trifoliata) that exhibit typical symptoms of Psorosis disease have been detected and collected from five different orchards located in the regions of Mitidja (Algeria). All collected samples were subjected to biological testing and double antibody sandwich ELISA (DASELISA) as diagnostic tests. The serological method, specifically DAS-ELISA, yielded positive results on varieties from the prospect station, indicating the presence of psorosis in Algeria. The typical symptoms of psorosis were observed through the indexed seedlings and grafts.